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人胚腎細胞;293 HEK-293

更新時間:2016-06-15

簡要描述:

人胚腎細胞;293 [HEK-293],開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人胚腎細胞;293 [HEK-293]
形態特性 上皮樣
生長特性 貼壁生長 
特征特性人胚腎細胞;293 [HEK-293]早期報道中指出該細胞基因組中含有腺病毒5(Ad5)基因組的左側端和右側端的DNA,但是現在明確了只存在其左側端的DNA。經過對Ad5的插入點的克隆測序發現,Ad5的1~4344位線性核苷酸整合入細胞染色體19q13.2。該細胞為人類腺病毒載體擴增的宿主??杀磉_異常的玻連蛋白的細胞表面受體,由整合素β1亞單位和玻連蛋白受體α-v亞單位組成。生物安全級別為2級。
培養條件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS 
傳代方法 1:10~1:20傳代,每2~3天換液1次。
傳代情況 P20
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
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