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人包皮成纖維細胞;HFF

更新時間:2016-06-21

簡要描述:

人包皮成纖維細胞;HFF,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人包皮成纖維細胞;HFF
形態特性 成纖維細胞樣 
生長特性 貼壁生長 
特征特性 人包皮成纖維細胞;HFF從新生兒包皮中分離建立,可用作飼養層細胞,用于支持胚胎干細胞的生長或維持胚胎干細胞的未分化狀態。
培養條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS 
傳代方法 1:2傳代;3~4天1次。
傳代情況 P18
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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